2011年11月25日，山东省农科院董合忠、孔祥强利用NMT在Journal of Experimental Botany 上发表了标题为Effects of non-uniform root zone salinity on water use Na+ recirculation, and Na+ and H+ flux in cotton的研究成果。
- 期刊：Journal of Experimental Botany
- 标题：Effects of non-uniform root zone salinity on water use Na+ recirculation, and Na+ and H+ flux in cotton
A new split-root system was established through grafting to study cotton response to non-uniform salinity. Each root half was treated with either uniform (100/100 mM) or non-uniform NaCl concentrations (0/200 and 50/150 mM).
In contrast to uniform control, non-uniform salinity treatment improved plant growth and water use, with more water absorbed from the non- and low salinity side. Non-uniform treatments decreased Na+ concentrations in leaves. The [Na+] in the ‘0’ side roots of the 0/200 treatment was significantly higher than that in either side of the 0/0 control, but greatly decreased when the ‘0’ side phloem was girdled, suggesting that the increased [Na+] in the ‘0’ side roots was possibly due to transportation of foliar Na+ to roots through phloem.
Plants under non-uniform salinity extruded more Na+ from the root than those under uniform salinity. Root Na+ efflux in the low salinity side was greatly enhanced by the higher salinity side. NaCl-induced Na+ efflux and H+ influx were inhibited by amiloride and sodium orthovanadate, suggesting that root Na+ extrusion was probably due to active Na+/H+ antiport across the plasma membrane.
Improved plant growth under non-uniform salinity was thus attributed to increased water use, reduced leaf Na+ concentration, transport of excessive foliar Na+ to the low salinity side, and enhanced Na+ efflux from the low salinity root.
通过嫁接建立了一个新的分根系统，以研究棉花对盐度不均匀的响应。每个根部均用均一的（100/100 nonmM）或不均一的氯化钠浓度（0/200和50/150 mM）处理。
盐度不均匀的植物比盐度均匀的植物从根部挤出的Na+更多。低盐度一侧的根Na+流出量被高盐度一侧大大增强。NaCl诱导的Na+流出和H+流入被阿米洛利和原钒酸钠抑制，这表明根Na+的挤出可能是由于跨质膜的活性Na+ / H+逆向转运所致。
Effects of non-uniform (0/200 mM and 50/150 mM NaCl) and uniform (0/0, 50/50, 100/100, and 150/150 mM NaCl) root zone salinity on net Na+ ﬂuxes in roots of cotton at 1 d (A) and 7 d (B) after treatment. The data are main ﬂuxes of Na+ within the measuring periods (15 min). Values are means 6SE (n¼6). Bars with different letters (a, b, and c) differ signiﬁcantly at P < 0.05.