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活性氧创新科研平台成果:中农学者阐明提高籼稻愈伤组织培养力的分子机制

转自中关村旭月非损伤微测技术产业联盟

【Nature Commun】 中国农大付永彩组阐明提高籼稻愈伤组织培养力的分子机制


期刊:Nature Communications
主题:中农学者阐明提高籼稻愈伤组织培养力的分子机制
标题:A common wild rice-derived BOC1 allele reduces callus browning in indica rice transformation
影响因子:11.878
检测指标:H2O2流速
检测样品:水稻愈伤组织
H2O2流实验处理方法:不同水稻传代培养21天的愈伤组织
H2O2流实验测试液成份:0.1mM KCl,0.1mM CaCl2、0.1mM MgCl2、0.5mM NaCl,0.3mM MES,0.2mM Na2SO4,pH 6.5
作者:中国农业大学付永彩、张坤、苏晶晶、徐敏

英文摘要

Callus browning, a common trait derived from the indica rice cultivar (Oryza sativa L.), is a challenge to transformation regeneration.

Here, we report the map-based cloning of BROWNING OF CALLUS1 (BOC1) using a population derived from crossing Teqing, an elite indica subspecies exhibiting callus browning, and Yuanjiang, a common wild rice accession (Oryza rufipogon Griff.) that is less susceptible to callus browning.

We show that BOC1 encodes a SIMILAR TO RADICAL-INDUCED CELL DEATH ONE (SRO) protein. Callus browning can be reduced by appropriate upregulation of BOC1, which consequently improves the genetic transformation efficiency. The presence of a Tourist-like miniature inverted-repeat transposable element (Tourist MITE) specific to wild rice in the promoter of BOC1 increases the expression of BOC1 in callus.

BOC1 may decrease cell senescence and death caused by oxidative stress. Our study provides a gene target for improving tissue culturability and genetic transformation.

中文摘要(谷歌机翻)

愈伤组织褐变是源自rice稻品种(Oryza sativa L.)的常见性状,对转化再生提出了挑战。

在这里,我们报告了基于图谱的克隆CALLUS1(BOC1)的克隆,该群体来自穿越易感褐变的优良in稻亚种特清和较不易感的常见野生稻种(Oryza rufipogon Griff)杂交的jiang江。愈伤组织褐变。

我们显示,BOC1编码类似到自由基诱导的细胞死亡一(SRO)蛋白。通过适当上调BOC1可以减少愈伤组织的褐变,从而提高遗传转化效率。BOC1启动子中特有的野生稻特有的类似于游客的微型反向重复转座因子(Tourist MITE)的存在增加了BOC1在愈伤组织中的表达。

BOC1可以减少氧化应激引起的细胞衰老和死亡。我们的研究为改善组织可培养性和遗传转化提供了基因靶标。

 

文章链接:https://www.nature.com/articles/s41467-019-14265-0

 

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