微生物NMT标准化方案 | 中关村认证实验标准

叶肉组织细菌侵染Ca²⁺流实验-非损伤微测技术

精确监测金鱼藻吸放氧-非损伤微测技术

微藻检测-非损伤微测技术

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202110224

1）Extracellular polymeric substrates of Chlorella vulgaris F1068 weaken stress of cetyltrimethyl ammonium chloride on ammonium uptake

2）Microalgae in Microwell Arrays Exhibit Differences with Those in Flasks: Evidence from Growth Rate, Cellular Carotenoid, and Oxygen Production

3）Paxillus involutus-Facilitated Cd²⁺ Influx through Plasma Membrane Cd²⁺-Permeable Channels Is Stimulated by H₂O₂ and H⁺-ATPase in Ectomycorrhizal Populus × canescens under Cadmium Stress

4）Hexavalent chromium induced oxidative stress and apoptosis in Pycnoporus sanguineus

5）The possible role of bacterial signal molecules N-acyl homoserine lactones in the formation of diatom-biofilm (Cylindrotheca sp.)

6）Physiological fluxes and antioxidative enzymes activities of immobilized Phanerochaete chrysosporium loaded with TiO₂ nanoparticles after exposure to toxic pollutants in solution

7）Mechanisms underlying turgor regulation in the estuarine alga Vaucheria erythrospora (Xanthophyceae) exposed to hyperosmotic shock
Ca²⁺ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp. C2

8）Responses of Phanerochaete chrysosporium to Toxic Pollutants: Physiological Flux, Oxidative Stress, and Detoxification

9）Surface tip-to-base Ca²⁺ and H⁺ ionic fluxes are involved in apical growth and graviperception of the Phycomyces stage I sporangiophore

10）Electrical Phenotypes of Calcium Transport Mutant Strains of a Filamentous Fungus, Neurospora crassa

11）Osmotic adjustment and requirement for sodium in marine protist thraustochytrid

12）Ion transport and osmotic adjustment in Escherichia coli in response to ionic and non-ionic osmotica

13）Cyclopropane fatty acids improve Escherichia coli survival in acidified minimal media by reducing membrane permeability to H⁺ and enhanced ability to extrude H⁺

14）Turgor regulation in the osmosensitive cut mutant of Neurospora crassa

15）Ionic currents and ion fluxes in Neurospora crassa hypha

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202110225

Cd²⁺、Cu²⁺、Pb²⁺、NH₄⁺、O₂、H⁺、 K⁺、 Na⁺、Ca²⁺

1）O₂生理功能概述

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202110226

2）科研案例

以小球藻为材料，研究了其对微培养（5种不同的微孔深度，四种直径水平）的细胞反应。结果表明，O₂的流速随着深度从10µm增加到100µm逐渐升高，但随着深度加深到200µm，氧气流速迅速降低，这实际上是细胞生理学和密度的综合结果。综上所述，直径/深度比约为1的微孔中的细胞具有最高的比生长率和氧流量。模拟还表明，在直径与深度比较高的微孔中，传质效果更好。

Zhang P, Xiao Y, Li Z, Guo J, Lu L. Microalgae in Microwell Arrays Exhibit Differences with Those in Flasks: Evidence from Growth Rate, Cellular Carotenoid, and Oxygen Production. Front Plant Sci. 2018;8:2251. Published 2018 Jan 10. doi:10.3389/fpls.2017.02251

202110227