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NMT历史上的今天丨ENVIRON EXP BOT 铵营养与Cd胁迫关系 文章发表

转自中关村旭月非损伤微测技术产业联盟

NMT历史上的今天

2012年8月23日,广东农科院王旭、王富华、吴志超利用NMT在Environmental and Experimental Botany上发表了标题为Increasing ammonium nutrition as a strategy for inhibition of cadmium uptake and xylem transport in rice (Oryza sativa L.) exposed to cadmium stress的研究成果。

 

  • 期刊:Environmental and Experimental Botany
  • 主题:增加铵营养作为抑制镉胁迫下水稻(Oryza sativa L.)镉吸收和木质部运输的策略
  • 标题:Increasing ammonium nutrition as a strategy for inhibition of cadmium uptake and xylem transport in rice (Oryza sativa L.) exposed to cadmium stress
  • 影响因子:3.666
  • 检测指标:Cd2+流速
  • 检测部位:水稻根部根毛区(距离根尖700μm)
  • Cd2+流实验处理方法:8日龄的水稻,由不同的NO3- / NH4+比例(1:0,2:1,1:1,1:2,0:1)和三种Cd水平(0,1,5μM CdCl2)组成处理组
  • Cd2+流实验测试液成份:0.03 mmol L-1 CdCl2, 0.1 mmol L-1 CaCl2, 0.1 mmol L-1 KCl, 0.3 mmol L-1 MES, pH 5.5,NO3/NH4+ (1:0, 2:1, 1:1,1:2, 0:1)
  • 作者:广东农科院王旭、王富华、吴志超

英文摘要

Nitrogen (N) speciation could influence the efficiency of Cd accumulation by plants grown in Cd-contaminated areas. However, limited information has been reported on how the application of N speciation to rice affects the Cd accumulation.

Here, we investigated the physiological and genetic mechanisms involved in Cd uptake by roots, xylem translocation and subsequent Cd accumulation in rice (Oryza sativa L.) affected by different NO3/NH4+ ratios under low-and-high Cd stress. Results showed that both N speciation and Cd stress affected plant growth, with high NH4+-N ratios treatments having higher tissue biomass, and high Cd treatment reducing tissue biomass. The Cd concentrations in shoots and roots were reduced with the increasing NH4+-N ratios (except for the full NO3-N treatment), but did not affect the Cd translocation factor (TF).

The total Cd accumulation of the whole plant were also reduced with the high NH4+-N ratios (NO3/NH4+, 1:2 and 0:1) on the low Cd treatment due to the decline both in the Cd accumulation of shoots and roots. Non-invasive Micro-test Technology(NMT) showed that the net Cd2+ influxes at the root hair zone were inhibited with the increasing NH4+-N ratios in absence or pretreatment with different NO3/NH4+ ratios.

Additionally, the Cd concentrations in xylem sap were also displayed a decline trend with the increasing NH4+-N ratios under two dose Cd treatments. Furthermore, the gene expression related to Cd uptake (OsIRT1 and OsNRAPM5) and transport (OsHMA2) in roots showed the familiar tendencies with those of Cd uptake and transport at the physiological level treated with different NO3/NH4+ ratios under two dose Cd treatments. It is concluded that increasing ammonium nutrition contributes to the inhibition of Cd uptake, xylem transport and subsequent accumulation in rice.

 

中文摘要(谷歌机翻)

氮(N)物种形成可能影响Cd污染地区植物生长的Cd积累效率。然而,关于如何将N形态施用于水稻影响Cd积累的报道有限。

在这里,我们调查了在低和高Cd胁迫下受不同NO3/NH4+ 比例影响的水稻(Oryza sativa L.)根系吸收Cd,木质部易位和随后Cd积累的生理和遗传机制。结果表明,N物种和Cd胁迫均影响植物生长,NH4+-N比例处理组织生物量较高,Cd处理量较高,组织生物量较低。随着NH4+-N比值的增加,芽和根中的Cd浓度降低(除了完全的NO3-N处理),但不影响Cd转运因子(TF)。

由于芽和根中Cd积累量的下降,低Cd处理的NH4+-N比值(NO3-/NH4+,1:2和0:1)也降低了整株植物的总Cd积累量。 非损伤微测技术(NMT)显示,在不存在或用不同的NO3/NH4+ 比率预处理时,随着NH4+-N比率的增加,根毛发区域的净Cd2+流入受到抑制。

此外,在两剂Cd处理下,随着NH4+-N比值的增加,木质部汁液中的Cd浓度也呈下降趋势。此外,在两种剂量的Cd处理下,与Cd吸收(OsIRT1和OsNRAPM5)和转运(OsHMA2)相关的基因表达显示出与在不同NO3/NH4+ 比率下处理的生理水平下Cd吸收和转运相关的基因表达。结论是,增加铵营养有助于抑制水稻的Cd吸收,木质部运输和随后的积累。

Effect of different NO3/NH4+ ratios (A and B) or pretreatment with different ratios of NO3/NH4+ (C and D) on net Cd2+ influx at root hair zone by Non-invasive Micro-test Technology(NMT). Each point represents the mean values of Cd2+ influx of six roots from six individual plants during the time of 6th-10th minutes. Bars with different letters mean significantly statistical differences followed by one-way ANOVA analysis (LSD test, P < 0.05, n=6). 'preCK' indicates 'pretreatment of the rice seedlings with NO3/NH4+ (1:1)'.

文章链接:https://www.sciencedirect.com/science/article/abs/pii/S0098847218307871?via%3Dihub

 

 

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