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NMT历史上的今天丨ENVIRON EXP BOT 盐胁迫 文章发表

转自中关村旭月非损伤微测技术产业联盟

NMT历史上的今天

2017年11月18日,三峡大学何正权、中国林业科学研究院亚热带林业研究所邱文利用NMT在Environmental and Experimental Botany 上发表了标题为Overexpression of cysteine protease gene from Salix matsudana enhances salt tolerance in transgenic Arabidopsis的研究成果。

 

  • 期刊:Environmental and Experimental Botany
  • 主题:松柳半胱氨酸蛋白酶基因的过表达增强了转基因拟南芥的耐盐性
  • 标题:Overexpression of cysteine protease gene from Salix matsudana enhances salt tolerance in transgenic Arabidopsis
  • 影响因子:4.369
  • 检测指标:Na+、H+流速
  • 通讯作者:三峡大学何正权、中国林业科学研究院亚热带林业研究所邱文

英文摘要

Salix matsudana is a salt-tolerant arbor tree species. We are interested in understanding its mechanisms of salt-tolerance.

Here, we isolated a full-length 1080-bp cDNA for a salt stress-responsive cysteine protease gene from S. matsudana, designated SmCP. The deduced 359-amino-acid SmCP protein was essentially identical to cysteine proteases of other plant species and was predicted to contain inhibitor-I29 and peptidase-C1A domains with three active catalytic sites found in eukaryotic cysteine proteases.

Expression profile analysis revealed that SmCP expression is constitutive and overexpression in roots takes place under salt stress. Expression of SmCP in Escherichia coli cells led to enhanced salinity tolerance. In addition, transgenic Arabidopsis thaliana lines overexpressing SmCP displayed enhanced tolerance to salt stress, with increased germination rates, antioxidant enzyme (superoxide dismutase) activity, chlorophyll content and ion flux in the root, and reduced malondialdehyde (MDA) levels and electric conductivity compared with control plants.

These results indicate that SmCP likely plays an important role in salt tolerance in S. matsudana.

中文摘要(谷歌机翻)

柳柳是耐盐的乔木树种。我们有兴趣了解其耐盐性机制。

在这里,我们从S. matsudana,命名为SmCP的盐胁迫响应半胱氨酸蛋白酶基因中分离出全长1080 bp cDNA。推导的359个氨基酸的SmCP蛋白与其他植物物种的半胱氨酸蛋白酶基本相同,并且预计包含在真核半胱氨酸蛋白酶中具有三个活性催化位点的抑制剂I29和肽酶C1A域。

表达谱分析表明,SmCP表达是组成性的,根系在盐胁迫下发生过表达。 SmCP在大肠杆菌细胞中的表达导致盐度耐受性增强。此外,过表达SmCP的转基因拟南芥品系显示出更高的耐盐胁迫性,与发芽率,抗氧化酶(超氧化物歧化酶)活性,根中的叶绿素含量和离子通量相比增加,丙二醛(MDA)含量和电导率降低。控制植物。

这些结果表明,SmCP可能在松材线虫的耐盐性中起重要作用。

Fig. 12 Na+/H+ flux. Two-week-old seedlings were treated with 150 mM NaCl for 24 h. The roots were then used for flux measurements. WT: wild-type A. thaliana plants; CYS-1–CYS-3: SmCP-transformed A.thaliana plants. (A, B) Each point is the mean of five to six individual plants. (C, D) Average values of all the points in (A) and (B), respetively.Different letters indicate significant differences in comparisons (P < 0.05)

文章链接:https://www.sciencedirect.com/science/article/abs/pii/S0098847217302915?via%3Dihub

 

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