2015年12月27日，中科院遗传发育所童依平、何雪用NMT在Plant Physiology上发表了标题为The Nitrate-Inducible NAC Transcription Factor TaNAC2-5A Controls Nitrate Response and Increases Wheat Yield的研究成果。
- 期刊：Plant Physiology
- 标题：The Nitrate-Inducible NAC Transcription Factor TaNAC2-5A Controls Nitrate Response and Increases Wheat Yield
Nitrate is a major nitrogen resource for cereal crops; thus, understanding nitrate signaling in cereal crops is valuable for engineering crops with improved nitrogen use efficiency. Although several regulators have been identified in nitrate sensing and signaling in Arabidopsis (Arabidopsis thaliana), the equivalent information in cereals is missing.
Here, we isolated a nitrate-inducible and cereal-specific NAM, ATAF, and CUC (NAC) transcription factor, TaNAC2-5A, from wheat (Triticum aestivum). A chromatin immunoprecipitation assay showed that TaNAC2-5A could directly bind to the promoter regions of the genes encoding nitrate transporter and glutamine synthetase. Overexpression of TaNAC2-5A in wheat enhanced root growth and nitrate influx rate and, hence, increased the root’s ability to acquire nitrogen. Furthermore, we found that TaNAC2-5A-overexpressing transgenic wheat lines had higher grain yield and higher nitrogen accumulation in aerial parts and allocated more nitrogen in grains in a field experiment.
These results suggest that TaNAC2-5A is involved in nitrate signaling and show that it is an exciting gene resource for breeding crops with more efficient use of fertilizer.
D and E, Nitrate inﬂux rates at the root tip surface (D) and expression levels of nitrate transporters in whole seedlings (E). Seedlings were grown for 4 d on solid medium that contained 6 mM nitrate. The roots were used to measure nitrate ﬂux rate in the measuring solution that contained 1 mM nitrate. Data are means 6 SE for six plants. Whole seedlings were used to analyze the expression of nitrate trans-porters. The relative expression levels were normalized to the expression of AtACTIN2 (AtACT2). Data are means 6 SE of three replicates. Asterisks indicate that the difference between the means of the wild type and the transgenic lines was signiﬁcant at the P , 0.05 (*) and P , 0.01 (**) levels.